TH and PHA testing

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nck21

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Oct 29, 2007
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696
Location
Salina, Kansas
We are going to test some cows and have red top tubes from SEK for biopryn. Can I use these? Are they the same as purple top tubes? Thanks
 

knabe

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Feb 7, 2007
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Location
Hollister, CA
they may not have EDTA in them.  you can tell the purple tops have EDTA in them as there is a small amount of EDTA liquid which keeps the blood from clotting.

i'm not sure, but the red one's may have blood clotters in them.  look up the part number/manufacturer and google it and see what it is for.

your vet should have some purple tops on had pretty much all the time.
 

DL

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Jan 29, 2007
Messages
3,622
nck21 said:
We are going to test some cows and have red top tubes from SEK for biopryn. Can I use these? Are they the same as purple top tubes? Thanks

short answer NO
to get the DNA you need the white cells (ie cells with a nucleus) and you need them floating free so they can be removed

red top tube is a clot tube - -it has nothing in it - so the blood naturally clots -purpose is to get the serum (yellow fluid above the clot) - all the cells (white, red and platelets- are in a big gemish in the clot; blood being able to clot is good so you don't bleed to death but bad for getting DNA

serum is used to test for hormones, electrolytes etc

whole blood for complete blood counts etc - where the cells are floating free

Purple top tubes have the anit coagulant EDTA in them so the bolld doesn't clot - you need to rock them back and forth after you draw the blood so the EDTA gets mixed in

ps need to use separate needle and syringe (If that is how you draw blood) or separate needle and tube for each critter
 

jbzdad

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Joined
Jan 21, 2009
Messages
783
Location
southwestern Kansas
got my result back from Igenity in 3 weeks, they did the tests on hair roots!yes they are accepted by AMAA, my vet got the test cards from Igenity
 
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