Breeding Dilemma - In About 12 Hours

I have a situation I've not had before and need advise and/or to know if anyone else has had this happen.

I had a cow in standing heat early morning on May 16 and bred her late that evening.  I usually stick on a second Estrotect patch at about 17 or 18 days after A.I. to help me check heats, but on day 16 I thought she was acting like she was coming into cycle so I stuck a patch on her.  She acted a bit bullish for that evening but nothing rode her and her patch remained un-rubbed. On June 14th, 29 days post breeding, I pulled a blood sample for BioPRYN testing.  This past Thursday the sample report came back as "pregnant" with a score of .21.

Today, 19 days from when I thought she was displaying signs of heat,  she is standing for one of my bull calves (about a 450 lb calf) and acting bullish with the other cows.  From all classic signs she is in standing heat.

The main points:

-  She calved on Feb. 25th, so she was well past any postpartum days requirements at over 110
    days.
-  She was the only sample I pulled or sent for sampling.  I have not preg-checked any other
    cattle to date so no chance of mixing samples or mislabeling.

- The test is 95% for positive outcomes, with most negatives a result of too-early sampling or an embryo lost later after testing.  Her blood was just tested last Tuesday / Wednesday so I think embryo loss and heat in the last four days unlikely, and I already mentioned she is over 110 days postpartum.

I don't want to leave her open, but I also do not want to A.I. a pregnant cow.  Not sure what to do.  Any help appreciated.

i would almost 99% say she is bred

If she is only standing for a calf I would not get too excited. Any other signs of heat, other then the calf riding her? Is she sniffing and trying to ride other cows? Any gel discharge? Walking around bawling?

frostback said:

If she is only standing for a calf I would not get too excited. Any other signs of heat, other then the calf riding her? Is she sniffing and trying to ride other cows? Any gel discharge? Walking around bawling?

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She's throwing her head and pushing around on other cows - just like would would expect to see with one coming in.  When it cools down this evening I'll see what the other cows are doing with her.  That little bull calf has been quite the heat detector this spring though...    i just can't imagine a false positive on that blood sample.

If she was pregnant and early embryonic death occured, the test could still show up positive, have her manually checked.

Waltoncattle said:

If she was pregnant and early embryonic death occured, the test could still show up positive, have her manually checked.

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That's happened here.  Cow didn't come back in heat the first cycle after breeding.  Blood test pregnant, but then standing again after the positive test. 

Yes, if you go to the BioPryn website, they recommend a recheck at 90 days.