IVF Embryos TH X TH

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knabe

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technically it's possible.

whether someone has licensed the markers to do this and whether they have the skill to tease of number of cells from cluster without affecting cluster and have enough material or knowhow from a minimal number of cells is the question.

basically screening embryo's.

personally, I would edit heatwave.

there realistically is probably not an alternative to the mutation.
 

BM

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Jan 16, 2015
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Clark Club Calves said:
I see that they are selling IVF embryos mating one TH carrier to another TH carrier.  Can they pick out the embryos that are affected by TH and leave the carriers?

I don't think I've done any TH embryos, but routinely do embryo biopsy testing for Cross Country, including gender.  There is error though that the owner needs to be aware of.  The process involves a pre-amplification step of the entire genome.  I won't go into too much detail but it's possible that the region we need to look at for each test could not amplify enough for the test to work at all or for one allele not to amplify.  So for example, I genotype a female embryo and it ends up a bull calf, the Y chromosome didn't get amplified enough to show up.  On the other hand if I do get a carrier result or in the gender case an X and Y, that means both alleles did amplify and are present.  So in the case you are talking about there could be some THA embryos that are really THC because the free allele was just missing from the initial amplification step... make sense??? Nothing I can do about it, it's just the fact that you are starting out with so little DNA.  So the recommendation is that all animals are tested again after they are born.  I haven't gotten a lot of reports of incorrect tests, but it does happen.  I don't know much about the biopsy part.  I do know that it can decrease viability somewhat, but I have no idea on the stats for that. 
It's not all that common though, I'm not sure what the charges are for the biopsies, but the pre-amplification step is pretty costly per sample, and then you have to retest after. 
and Knabe - if someone gave me a cell line and the $ to reverse the mutation in heatwave, I think we could get it done fairly easily with an allele replacement.
 
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