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Offline CRS

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Post freezing morphology
« on: March 25, 2020, 01:44:38 AM »
Were collecting a young bull right now, just over 10 months, hes freezing high quality semen, but not passing post thaw morphology. Any recommendations on getting his post thaw count up? 
Life is tuff, but it is tougher when you are stupid.

Offline Steve123

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Re: Post freezing morphology
« Reply #1 on: March 25, 2020, 08:13:03 AM »
It could be that you are low on heat shock protein.  This is a cut from the first paragraph in the section: Molecular Markers Of Sperm Freezability

Holt et al. (54) claim that lower concentrations of heat shock protein A8 (HSPA8) in freezing media cause reduced post-thaw sperm viability, whereas higher concentrations improve plasma membrane integrity.

Here is the full paper you can share with your technician to maybe trouble shoot a cause.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6736622/

Offline CRS

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Re: Post freezing morphology
« Reply #2 on: March 25, 2020, 11:18:25 AM »
Thanks, Ill give it a read after bit when Im doing some social distancing
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Offline CAB

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Re: Post freezing morphology
« Reply #3 on: March 25, 2020, 06:50:46 PM »
It would be more common to not freeze than to freeze at this age. I'd wait 2 to 3 months and try him again.

Offline hntwhitetail

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Re: Post freezing morphology
« Reply #4 on: March 26, 2020, 07:30:03 PM »
Were collecting a young bull right now, just over 10 months, hes freezing high quality semen, but not passing post thaw morphology. Any recommendations on getting his post thaw count up?

Was wondering what Mil your stud is collecting him at.

Offline CRS

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Re: Post freezing morphology
« Reply #5 on: March 27, 2020, 05:10:58 PM »
He's producing almost 5 milliliters and they are putting it in 5 million cells per unit.
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Offline hntwhitetail

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Re: Post freezing morphology
« Reply #6 on: March 27, 2020, 06:32:16 PM »
He's producing almost 5 milliliters and they are putting it in 5 million cells per unit.

Just wondering if you requested that mix.  I thought most put in 10mil per unit.  I'm getting ready to have some collected and will be @ 45mill cells per unit.  I think 1 of the large angus pack theirs @ 40 per unit.  $40 for 40mil a straw

Offline Medium Rare

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Re: Post freezing morphology
« Reply #7 on: March 27, 2020, 08:16:11 PM »
Herbster advertises 40 mill for $40.

Offline CRS

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Re: Post freezing morphology
« Reply #8 on: March 27, 2020, 08:19:38 PM »
Very possible I am wrong.  He is at Hawkeye, so whatever their standard is.  His is making 40-50 units per collection now
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