We have had knots like you are talking about from injection sites, we have one in a group of heifers we ai'd now. I know this knot is an injection site, some of the sub Q shots seem to bubble up under the skin and stay for quite awhile, ideally I guess you would give the shot and rub the area for a little bit to make sure it spreads out a little. All I know is we run them through and vaccinate or whatever and next thing you know they have a knot at the injection site, doesn't happen very often but it has happened.