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outcast < Stinger <
if the lines are DNA, and you need one set of primers (which help amplify dna in a specific region along with dna polymerase and other mumbo jumbo) indicated by the arrows, you have two sets of primers. the lab probably has to run the sets of primers independently to not get curious results, so they probably need to know the background of the animal in question,or just run both sets all the time, which, i guess in theory, you could get an extremely rare crossover event BETWEEN the two regions and get both defects in the same allele. you could also get both defects in a dead calf probably with different backgrounds. if you ran both sets, maybe the designed fragment lengths are similar and therefore make scoring difficult.
more on the arrows. the arrows indicate amplification of that specific region of dna. this amplified product is then digested by a dna sequence specific enzyme to yield either one or two products. if you have one size fragment of the unaffected length, the calf is safe. if you have two different size bands, you have a carrier. i guess you could test a dead calf and you might get a twice as bright bands as the yield would be double.
this is probably gobbledygook.
bottom line, there is NO CONSPIRACY with this test. the only conspiracy is the need to keep this defect going. again, i still say, that if there is diversity in this gene and the PHA gene that has merit, breed on a very selective and designed basis. if not, it's terminal in my book.
i didnt' put any ratio's to have dusty correct my math as he does things the correct way by doing the square. i wish more would use this parable to breed for other traits. oops that would be line breeding and no one wants to do that, except for maybe 3 or 4 people on the planet including myself. of that number, i'm the loser who hasn't done anything yet. outcast
