XBAR $25-$50 does not determine what I do. I stated in my post it was more important to get the heifer bred than the cost of an additional unit of sexed semen. As usual, you add nothing to the conversation.
I used one unit of conventional semen. I was fine with the usual risk on conventional, just wasn't comfortable with sexed if the conception rate is lower. I didn't feel two units conventional were necessary since I knew when she came in heat and when she went out. I am kind of particular on...
I have a heifer that I want to breed using sexed semen. I'm told sexed semen will have a little lower conception rate than conventional. She was cycling regularly, until I was ready to breed her about 45 days ago. Now she's standing. I hate to used sexed and lessen my chances of sticking...
If I remember the literature correctly, lutalyse works in the day 5 to day 13 window of a 21 day cycle. It appears the cow is in day 3 or day 4. I can't give you odds, but I know that trying to bring cows in heat outside that window probably doesn't work. However, Murphy's Law may apply -...
Don't know who's doing your invitro work, but the one I am familiar with, prefers thawing conventional semen and then sorting (sexing) it. They have a better success rate than using frozen sexed semen for fertilization. I didn't ask, but I assume there is less total stress on the wigglers...
If there was liquid in it, it should be fine. Semen doesn't have to covered with LN. You're right your lack of vapor when you opened it is due to your low air temp.